【Background】The western flower thrips (Frankliniella occidentalis) was introduced into China in 2003, and its occurrence and damage is increaseing. Chemical control is currently widely used but unsustainable. This work is aimed at screening and identification of potentially resistant germplasm resources. 【Method】Juvenile seedlings of different Capsicum annuum cultivars were infested with 1st nympha instar F.occidentalis in glasshouse, and the index of damage was calculated by revised damaged symptoms and graded standard. The correlation between the activity of plant defense enzymes and resistance levels was analyzed by the activity variation of calatase (CAT) and peroxidase (POD). 【Result】Two out of 20 tested cultivars, Xiangyan No.13 and Bola No.4 demonstrated high levels of resistance/tolerance, while the others had middle level of resistance/tolerance or were susceptible. Cv. Xingshu Lüjian the most susceptible cultivar. A positive relationship was observed between POD activity and resistance level on selected cultivars, but CAT did not seem to be related to resistence. 【Conclusion and significance】In the study, the method of evaluation and screening resistance/tolerance to western flower thrips (F.occidentalis) on different C.annuum cultivars at the juvenile seedlings stage in glasshouse was developed, which are proof of discovering the resistant plant germplasm resources and further effective control to western flower thrips.