【Background】The feeding choice of Bemisia tabaci Bbiotype can be affected by different tobacco varieties, but the specific mechanism is unclear. Electrical penetration graph (EPG) technique is an important method that can study feeding choice of piercingsucking insects on host plants, and it can analyze the feeding differences of B.tabaci on different tobacco varieties, through the electrical signals change generated by feeding of B.tabaci. 【Method】Feeding behaviors of female adults of B.tabaci Bbiotype on 6 tobacco varieties (cv.CB1, K326, Minyan 7, Yunyan 87, Yunyan 85 and Honghuadajinyuan) were observed by EPG technique. 【Result】The feeding behaviors of 18 female adults were effectively recorded , on each variety. 15, 15, 12, 13, 15 and 15 individuals reached phloem of cv.s CB1, K326, Minyan 7, Yunyan 87, Yunyan 85 and Honghuadajinyuan respectively, and among them, 13, 14, 8, 12, 14 and 15 individuals could ingest plant sap continuously. There was significant different in the percentage of nonprobing of female adults on different varieties. The percentage of nonprobing on Honghuadajinyuan was the smallest. Total duration of salivation into sieve elements (waveform E1) on cv.s Honghuadajinyuan and K326 were significantly shorter than those on cv.s Minyan 7 and Yunyan 87; The total duration of phloem ingestion (waveform E2) on Honghuadajinyuan were significantly longer than others; The times of all E2 periods on cv.s Minyan 7, Yunyan 87 and Yunyan 85 were significantly shorter than those on other three varieties. 【Conclusion and significance】Female adults of B.tabaci Bbiotype preferred cv.Honghuadajinyuan, and least preferred cv.s Minyan 7 and Yunyan 87 among the examined tobacco cultivars. Therefore, in Fujian Province where B.tabaci Bbiotype is present/ invading, planting tobacco cv.Honghuadajinyuan would be advantageous, and B.tabaci Bbiotyperesistant varieties such as cv.Minyan 7 and Yunyan 87 are more widely planted than currently.