【Background】Epiblema strenuana is an important natural enemy of Ambrosia artemisiifolia, it has rapidly spread to several adjacent Provinces such as Hubei, Jiangxi and Fujian since it was released into Linxiang in Hunan Province in 1993, and it to some extent has suppressed the spread and expansion of A.artemisiifolia population.【Method】To clarify occurrence and cold hardiness of E.strenuana in Hubei province, the field survey was conducted in the areas invaded by A.artemisiifolia Hubei Province in 2009, and the survival rate of overwintering old larvae was determined at different low temperatures.【Result】E.strenuana distributed mainly in the eastern region of Hubei Province. Overwintering adults occurred in late May to early June, the peak of 1st generation larvae were observed in late June to early July, and old larvae entered into diapause in late October. The results of survey in December showed that the insectinfected rate of A.artemisiifolia plants was 90%, the density was 313 larvae per 100 plants,and survival rate of larvae was 93.〖KG-*8〗3%. The supercooling point of overwintering old larvae was -26.4 ℃, and both survival rate of overwintering old larvae and survival time decreased significantly with the decreasing temperature.【Conclusion and significance】E.strenuana has widely distributed in Hubei Province, it can overwinter successfully in the areas invaded by A.artemisiifolia, thus it performs a sustainable control effect of A.artemisiifolia in Hubei Province.