Dwarf bunt of wheat, caused by Tilletia controversa Kühn (TCK), is an important quarantine disease in China. To determine the feasibility of electronic nose in rapid detection of TCK, wheat grains containing different numbers of TCK or T.foetia (Wallr.) Liro (TFL) teliopores (0, 100, 101, 102, 103, 104, 105 teliopores in 50 g wheat grains, respectively) were testified by using electronic nose. Data were analyzed by principal component analysis (PCA) and linear discriminant analysis (LDA). The results showed that wheat without TCK teliopores could be distinguished from those with TCK teliopores by using these 2 analysis methods. And it was also possible to distinguish 0, 100, 101, 102 and ≥103 TCK teliopores in 50 g wheat grains by using LDA. Moreover, TCK and TFL could be distinguished by using PCA. Therefore, this study laid a foundation for application of electronic nose in rapid detection of TCK in wheat grain.