In order to develop standards for safe use of acetamiprid on grapes, a supervised trial experiment which used highperform liquid chromatography were conducted to study the dynamics of degradation of acetamiprid in grape fruits and leaves. The halflives of acetamiprid 3% ME in grape leaves and berries were 3.[KG-*8]55~3.[KG-*8]93 days and 4.[KG-*8]14~5.[KG-*8]60 days, respectively. Being treated 3 times for every 7 days with acetamiprid 3% ME at recommended concentration of 22.[KG-*8]5 g·hm-2 (a.i.) and double concentration of 45 g·hm-2 (a.i.), the residue of acetamiprid in grape fruits 7 days later was <0.[KG-*8]2 mg·kg-1, and 14 days later <0.[KG-*8]1 mg·kg-1. The limit detection amount and concentration were 0.[KG-*8]5 ng and 0.[KG-*8]01 mg·kg-1 respectively. These values are lower than the relevant maximum residues limits of the United States, South Korea and Japan. The results suggested that acetamiprid 3% ME could be used in grape 1~3 times for every 7 days at a concentration of 22.[KG-*8]5 g·hm-2 (a.i.) with a safety interval of 7 days before harvest.