In recent years, DNA barcode technology make great help in identification of species, but there are some problems with the development of the technology. Minibarcode technology as a supplement to DNA barcoding has been proposed recently. This method amplifies a sequence which is much shorter than the cytochrome c oxidase subunit I sequence by universal primers to identify and classify species. As a new technology, its advantages include, such as amplifying the target gene for partly degenerated DNA samples, as well as a good solution to the diversity of environmental investigation in mixed samples and so on. However, the target gene of the new technology is a very short DNA fragment. Therefore, the genetic marker sequence contains limited information. So, there is a certain gap between the full barcode in the accuracy of identification. Here, based on previous studies, we make a brief summary of advantages and disadvantages of DNA minibarcode and its potential applications, especially to identification of the invasive species.